Despite significant progress, high-speed live-cell super-resolution studies remain limited to specialized optical setups, generally requiring intense phototoxic illumination. Here, we describe a new ...
Limited by the laws of physics, scientific discoveries and the advance of knowledge can be bound up in a series of slow proceedings. In microscopy, up until the early 21 st century, the diffraction ...
Image comparison of a fluorescently labeled BPAE cell, recorded with a widefield fluorescence microscope and a SRRF-Stream enabled iXon Life 888 EMCCD camera. A x63 objective was used, with further 2x ...
Super-resolution microscopy and single molecule fluorescence spectroscopy often require mutually exclusive experimental strategies optimizing either time or spatial resolution. While the measurement ...